mouse anti integrin α4 antibody  (Proteintech)

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 2. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopulation were isolated and sorted from ASCs by fluo- rescence-activated cell sorting. (A) The population of integrin α4 ＋ ASCs were about 5.11% and 70.7% in the pre- and postsorted ASCs, respectively. (B) Immunofluorescent staining revealed integrin α4 were robustly expressed in integrin α4 ＋ ASCs subpopulation. (C) Western blotting showed that the ex- pression of integrin α4 with 135 kDa was obviously more in integrin α4 ＋

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Isolation, FACS, Staining, Western Blot

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 4. Identification of Integrin α4 ＋/green fluorescent protein＋ (GFP＋) adipose-derived stem cells (ASCs) and incorporation of injected ASCs into the vasculature by immunofluorescent staining in infarcted myocardium 4 weeks posttransplantation. (A) Immunofluorescent staining was performed for GFP (green) and integrin α4 (red), and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue), co-localized expression of integrin α4 and GFP were detected in the hearts receiving integrin α4 ＋ ASCs subpopulation, and integrin α4 ＋ ASCs sub- population showed much higher engraftment than unfractionated ASCs. (B) Immunofluorescent staining was conducted for GFP (green) and von Willebrand Factor (vWF, red), and nuclei were stained with DAPI (blue), GFP-positive ASCs directly incorporated into vasculature.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Injection, Staining, Expressing

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 5. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopu- lation more robustly migrated and engrafted into the infartced my- ocardium after cell transplantation. (A) Four weeks posttranspla- ntation, green fluorescent protein-positive ASCs were more fre- quently detected in the hearts receiving integrin α4 ＋ ASCs sub- population than in the hearts undergoing unfractionated ASCs treatment. (B) The high reproducibility of the standard curve of re- al-time PCR. Serial dilution (10−2∼10−8) of DNA was made 7 times to construct the standard curve. Each pink circle corresponded to one dilution in one experiment. The blue solid line represented the regression analysis. (C) Four weeks after cell transplantation, in- tegrin α4 ＋ ASCs subpopulation treated rats showed the signifi- cantly greater ASC numbers per heart as compared to unfractio- nated ASCs treated rats. *p＜0.05 vs. unfractionated ASCs.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, Transplantation Assay, Serial Dilution, Construct

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 6. Integrin α4 + adipose-derived stem cells (ASCs) subpopulation more effectively reduced infarct size post-implantation in vivo. (A) Representative tomographic histograms of the 18F-fluorodeoxyglucose (FDG) positron emission tomography images at 4 weeks posttrans- plantation. Yellow colors were indicative of higher tracer uptake, while blue colors stood for lower tracer uptake. (B) Four weeks after transplantation, unfractionated ASCs transplantation markedly elevated the 18F-FDG uptake in apical-septal, apical-anterior, and apex seg- ments as compared with phosphate-buffered saline (PBS) control. Of importance, integrin α4 + ASCs subpopulation implantation further increased the 18F-FDG uptake in apical-septal, apical-inferior, and apex segments compared with unfractionated ASCs injection. (C) Representative transverse, coronal, and sagittal myocardial 18F-FDG images at 4 weeks after cell transplantation. The infarcted area can be visually detected as an area of low glucose metabolism. (D) Four weeks after transplantation, integrin α4 + ASCs subpopulation markedly reduced infarct size as compared to unfractionated ASCs and PBS control. (E) Integrin α4 + ASCs subpopulation significantly increased global 18F-FDG uptake compared with unfractionated ASCs and PBS control. *p＜0.05 vs. unfractionated ASCs. #p＜0.05 vs. PBS.

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, In Vivo, Positron Emission Tomography, Transplantation Assay, Saline, Control, Injection

Journal: International journal of stem cells

Article Title: Integrin α 4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction.

doi: 10.15283/ijsc22209

Figure Lengend Snippet: Fig. 7. Integrin α4 ＋ adipose-derived stem cells (ASCs) subpopulation more effectively improve cardiac fucntion post-implantation in vivo. (A, B) Four weeks after transplantation, integrin α4 ＋ ASCs subpopulation reduced left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) compared with unfractionated ASCs and phosphate-buffered saline (PBS) control. (C) Integrin α4 ＋

Article Snippet: A mouse anti-integrin α4 antibody was used to detect the grafted integrin α4+ ASCs. von Willebrand Factor (vWF), an endothelial cell marker, was identified by an mouse anti-vWF antibody (Proteintech).

Techniques: Derivative Assay, In Vivo, Transplantation Assay, Saline, Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: The Appendix Orchestrates T-Cell Mediated Immunosurveillance in Colitis-Associated Cancer

doi: 10.1016/j.jcmgh.2022.10.016

Figure Lengend Snippet: Blocking T-cell trafficking suppresses differences between appendectomized and not appendectomized mice. ( A–D ) To block T-cell trafficking to the colon, an anti-integrin α4-β7 antibody was administered twice a week (125 μg/100 μL of InVivoMAb anti-mouse LPAM-1, BE0034, Bio X Cell per injection) from first day of first DSS cycle to end of AOM/DSS protocol in mice that underwent appendectomy (n = 9) or sham laparotomy (n = 9). Intraperitoneal injections of sterile PPB (100 μL) were performed following the same chronology in control group (n = 8). At AOM/DSS protocol endpoint, the colons were taken. Dot plots showing comparisons between appendectomy and sham groups in terms of ( A ) macroscopic number of tumors, ( B ) microscopic number of tumors and median intratumor CD3+ ( C ) and CD8+ ( D ) T-cell densities (immunohistochemistry). ( E–H ) To block T-cell trafficking to the colon, FTY720 (60 μg/100 μL per injection, SML0700; Sigma-Aldrich) was administered to 8 mice after appendectomy and to 9 mice after sham laparotomy following the AOM/DSS protocol, and the frequency of injections was identical to that of the anti-integrin α4-β7 antibody. Intraperitoneal injections of sterile PPB (100 μL) were performed following the same chronology in control group (n = 8). Dot plots showing the comparisons between the appendectomy and sham groups in terms of ( E ) macroscopic number of tumors, ( F ) microscopic number of tumors and median intratumor CD3+ ( G ) and CD8+ ( H ) T-cell densities (immunohistochemistry). In all dot plots , the error bars represent the 25th, 50th (median), and 75th interquartile ranges. Comparisons of 3 groups were performed using the Kruskal-Wallis test. Comparisons of 2 groups were performed using Mann-Whitney test with 2-tailed P value. P value <.05 was considered statistically significant.

Article Snippet: Blocking T-cell trafficking suppresses differences between appendectomized and not appendectomized mice. ( A–D ) To block T-cell trafficking to the colon, an anti-integrin α4-β7 antibody was administered twice a week (125 μg/100 μL of InVivoMAb anti-mouse LPAM-1, BE0034, Bio X Cell per injection) from first day of first DSS cycle to end of AOM/DSS protocol in mice that underwent appendectomy (n = 9) or sham laparotomy (n = 9).

Techniques: Blocking Assay, Injection, Sterility, Control, Immunohistochemistry, MANN-WHITNEY